Download __EXCLUSIVE__ Free Pharma Guide For Pakistan



 
 
 
 
 
 
 

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Our previous studies demonstrated that tissue-type plasminogen activator (tPA) alters the distribution of plasminogen in both cultured tumor cells and in vivo. To determine if tPA could also alter the distribution of the plasminogen activator urokinase (uPA) in tumor cells, the distribution of uPA in R3230AC rat mammary adenocarcinoma cells was examined. Using an immunohistochemical analysis, we observed that uPA was concentrated in the perinuclear region of nonpermeabilized cells, which is also the subcellular region of tPA accumulation, and could only be partially redistributed to the cell membrane after uPA was permeabilized. Studies using a pulse-chase protocol demonstrated that the majority of uPA was internalized in the perinuclear region after 6 hr of chase and was found on the cell membrane after 12 hr of chase. To determine if tPA or uPA altered the distribution of their respective substrates, two plasminogen-fluorescein isothiocyanate (FITC) conjugates were prepared. These probes were used to examine changes in plasminogen distribution in response to tPA in R3230

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