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Using in vitro fibroblast and in vivo models of healing, transforming growth factor-beta3 (TGF-beta3) was identified as an active factor for the formation and maintenance of normal and pathological tendon. In this study, we hypothesized that TGF-beta3 is also involved in the process of fracture healing. We examined the protein expression of TGF-beta3 in a rat model of fracture healing (four animals were killed at each time point: 8 h, 24 h, 48 h, 3 days, 5 days, 7 days, and 11 days post-fracture) using immunohistochemical analysis. Observation of the histological samples showed that endothelial cells, fibroblasts, osteoblasts, and osteocytes were involved in the process of fracture healing. The duration of TGF-beta3 protein expression in the fracture callus was 8 h to 11 days after fracture. No TGF-beta3 expression was detected in the initial 8-h stage after fracture. After that, TGF-beta3 expression was detectable in fibroblasts and endothelial cells in the healing area at 24 h. At 48 h, TGF-beta3-positive cells increased in fibroblasts and endothelial cells and were localized in the bone cortex. However, at 5 days, and 7 days after fracture, TGF-beta3 expression was localized in proliferating fibroblasts, mononucleated stromal cells, and endothelial cells located in the fracture callus. At 11 days after fracture, TGF-beta3 protein was expressed in osteoblasts. These results suggested that TGF-beta3 is involved in the process of fracture healing during the initial 8-h stage as well as the later stages. These findings indicate that TGF-beta3 might be important for fracture healing and, thus, may have therapeutic potential for bone fracture healing.Q:

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